Part:BBa_K4947026:Design

G. max Chalcone Isomerase B2 Codon-Optimized CDS

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 43
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 43
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 43
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 43
  Illegal NgoMIV site found at 69
  Illegal AgeI site found at 148
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The codon optimization and domestication was done to improve recombinant expression in E. coli and enable restriction enzyme-based swapping of promoters and terminators, respectively.

Source

This gene homolog that encodes for CHR was chosen rationally after thorough literature review. CHI is an enzyme involved in the biosynthetic pathway of daidzein, converting isoliquiritigenin into liquiritigenin, both stable intermediates. The gene sequence was sourced from NCBI GenBank [1], and produced by Twist Bioscience.

References

1. https://www.ncbi.nlm.nih.gov/protein/Q53B70.1

2. View our contributions page (https://2023.igem.wiki/yale/contribution) for a spreadsheet of all our sources!

3. Liu, Q., Liu, Y., Li, G., Savolainen, O., Chen, Y., & Nielsen, J. (2021, October 19). De novo biosynthesis of bioactive isoflavonoids by engineered yeast cell factories. Nature News. https://www.nature.com/articles/s41467-021-26361-1

4. Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome. Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ. Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 10.1016/j.cell.2022.03.008. Epub 2022 Apr 1. 10.1016/j.cell.2022.03.008 PubMed 35366417